Dear colleagues with the development of reverse genetics systems recombinant viruses expressing reporter fluorescent or bioluminescent genes represent an excellent option to evaluate the dynamics of viral infection progression in both cultured cells and or validated animal models of infection. Keywords recombinant influenza a virus plasmid based reverse genetics virus rescue approaches reporter genes fluorescence luminescence replicating competent reporter expressing influenza a virus. To circumvent this requirement replication competent iav expressing an easily traceable reporter protein can be used here we discuss the development and applications of recombinant replication . We have generated a replication competent foot and mouth disease virus expressing nanoluciferase designated as nano fmdv nano fmdv is genetically stable the replication of nano fmdv can be monitored by bioluminescent methods. Notably the development of replicating competent reporter expressing viruses rcrevs has provided an excellent option to detect directly viral replication without the use of secondary labeling which represents a significant advance in virology
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